Assessment of Alpha-Tocopheryl Acetate and Metformin Hydrochloride as Independent Agents on Human Dermal Fibroblast Viability: Findings from MTT Assay

Authors

  • Nayla Majeda Alfarafisa Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran
  • Astrid Feinisa Khairani Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran https://orcid.org/0000-0001-7242-4791
  • Achadiyani Achadiyani Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran
  • Yoan Chou Master Program in Anti-aging and Aesthetic Medicine, Faculty of Medicine, Universitas Padjadjaran
  • Fitri Aulia Master Program in Anti-aging and Aesthetic Medicine, Faculty of Medicine, Universitas Padjadjaran
  • Muhammad Bintang Firdaus Faculty of Medicine Universitas Padjadjaran

DOI:

https://doi.org/10.15850/amj.v12n2.3954

Keywords:

Alpha-tocopheryl acetate, anti-aging, cell viability, metformin hydrocholride, MTT assay

Abstract

Background: Alpha-tocopheryl acetate (Vitamin E) and metformin hydrochloride have been tested as anti-aging compounds at various concentrations. This study aimed to identify the most effective concentrations of alpha-tocopheryl acetate and metformin hydrochloride in promoting the viability of human dermal fibroblasts (HDFs), a primary cell type in skin aging research.

Methods: HDFs were isolated using a mechanical isolation method and cultured under standard conditions. Cells were treated with varying concentrations of alpha-tocopheryl acetate and metformin hydrochloride as independents agents. After 48 hours of incubation, cell viability was measured using the MTT assay.

Results: Alpha-tocopheryl acetate had the highest HDF cell viability (107%) at a concentration of 50 μM. Metformin hydrochloride had the maximum HDF cell viability (158%) at 5 μM. However, the viability response varied across different concentrations for both agents, indicating that optimal dosing was essential for maximizing their effectiveness.

Conclusions: Alpha-tocopheryl acetate at 50 μM and metformin hydrochloride at 50 μM yield the highest viability of HDFs in vitro. These findings suggest potential roles for both agents in anti-aging skin therapies. Further research is recommended to explore their mechanisms of action and to optimize dosing strategies for clinical applications.

Author Biographies

Achadiyani Achadiyani, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran

Department of Biomedical Sciences, Division of Cell Biology

Fitri Aulia, Master Program in Anti-aging and Aesthetic Medicine, Faculty of Medicine, Universitas Padjadjaran

Graduate School of Master Program in Anti-aging and Aesthetic Medicine

Muhammad Bintang Firdaus, Faculty of Medicine Universitas Padjadjaran

Medical program

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Published

2025-06-27

Issue

Vol. 12 No. 2 (2025)

Section

Articles

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